Table 2 Oligonucleotides used for the mutations of Dlk2 GC-boxes
From: Characterization of a proximal Sp1 response element in the mouse Dlk2 gene promoter
PLASMID | OLIGONUCLEOTIDES | SEQUENCE |
|---|---|---|
pGL3Basic(-212/+1M1) | Sp1212/1M1F | 5'- GTGCGCAGCGGGGGTGGATATAA GGCGCAGTCGGTGCGGG -3' |
Sp1212/1M1R | 5'- CCCGCACCGACTGCGCCTTATAT CCACCCCCGCTGCGCAC -3' | |
pGL3Basic(-212/+1M2) | Sp1212/1M2F | 5'- GGGCGCAGTCGGTGCGGAATAA GCCGCGGCGGCGGGAGG -3' |
Sp1212/1M2R | 5'- CCTCCCGCCGCCGCGGCTTATT CCGCACCGACTGCGCCC -3' | |
pGL3Basic(-212/+1M3) | Sp1212/1M3F | 5'- GGCTCCGCCGGCACACGTTATT CCTGGCAGGGGGCCGAG -3' |
Sp1212/1M3R | 5'- CTCGGCCCCCTGCCAGGAATAA CGTGTGCCGGCGGAGCC -3' | |
pGL3Basic(+1/+177M4) | Dlk2MluI-400U | 5'- CCCGCGGCCAGCTAGGTTATTC CCCCCTCCCGCCCCC -3' |
Dlk2HindIII-1L | 5'- GGGGGCGGGAGGGGGGAATAA CCTAGCTGGCCGCGGG -3' | |
pGL3Basic(+1/+177M5) | Dlk2MluI-205U | 5'- AGGGGCGGCCCCCCTCAATAA CCCGGCCCACGTCCGTC -3' |
Dlk2HindIII-1L | 5'- GACGGACGTGGGCCGGGTTATT GAGGGGGGCCGCCCCT -3' | |
pGL3Basic(+1/+177M6) | Dlk2 Xho-212U | 5'-GCCCCCCTCCCGCCCCAATTAA CAGTCCGTCGGATCCG-3' |
Dlk2HindIII-1L | 5'- CGGATCCGACGGACTGTTAATT GGGGCGGGAGGGGGGCC -3' |