Gene symbol | Primer sequencea | Amplicon length (bp) | Annealing temperature (°C) | PCR efficiencyb/E | R |
---|---|---|---|---|---|
GAPDH | F: ACAGTCAGCCGCATCTTCTT R: GTTAAAAGCAGCCCTGGTGA | 127 | 57 | 1.001 | 0.999 |
ACTB | F: GGCGGCAACACCATGTACCCT R: AGGGGCCGGACTCGTCATACT | 205 | 62 | 1.06 | 0.95 |
TUBA1A | F: GCAACAACCTCTCCTCTTCG R: GAATCATCTCCTCCCCCAAT | 200 | 57 | 1.043 | 0.991 |
TUBB1 | F: CCTTGCAGCTGGAGAGAATC R: CTGTCGGGTTGAAAGAGAGC | 145 | 59 | 0.967 | 1.00 |
VIM | F: GACAGGATGTTGACAATGCG R: GTTCCTGAATCTGAGCCTGC | 135 | 59 | 1.03 | 0.998 |
K10 | F: GCCAACATCCTGCTTCAGAT R: TCACATCACCAGTGGACACA | 256 | 58 |