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Table 2 Sequences and related information of the primers

From: Selection of reference genes for gene expression studies in ultraviolet B-irradiated human skin fibroblasts using quantitative real-time PCR

Gene

symbol

Primer sequencea

Amplicon length

(bp)

Annealing temperature

(°C)

PCR efficiencyb/E

R

GAPDH

F: ACAGTCAGCCGCATCTTCTT

R: GTTAAAAGCAGCCCTGGTGA

127

57

1.001

0.999

ACTB

F: GGCGGCAACACCATGTACCCT

R: AGGGGCCGGACTCGTCATACT

205

62

1.06

0.95

TUBA1A

F: GCAACAACCTCTCCTCTTCG

R: GAATCATCTCCTCCCCCAAT

200

57

1.043

0.991

TUBB1

F: CCTTGCAGCTGGAGAGAATC

R: CTGTCGGGTTGAAAGAGAGC

145

59

0.967

1.00

VIM

F: GACAGGATGTTGACAATGCG

R: GTTCCTGAATCTGAGCCTGC

135

59

1.03

0.998

K10

F: GCCAACATCCTGCTTCAGAT

R: TCACATCACCAGTGGACACA

256

58

  
  1. a All primer sequences are given in the 5'→3' direction.
  2. b PCR efficiencies were determined by PCR DataMiner and confirmed by a dilution series experiment.