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Figure 5 | BMC Molecular Biology

Figure 5

From: TGFβ1 enhances MAD1 expression and stimulates promoter-bound Pol II phosphorylation: basic functions of C/EBP, SP and SMAD3 transcription factors

Figure 5

SP1 and C/EBPβ cooperate in MAD1 promoter reporter gene activation. A. HeLa cells were co-transfected with the -184 to +248 reporter gene construct and C/EBPβ and/or SP1 expression vectors as indicated. B. A dominant negative SP1 mutant (SP1dn) was co-expressed with C/EBPβ in HeLa cells and the activity of the -184 to +248 reporter gene construct was determined. C. and D. HeLa cells were transiently transfected with the indicated expression plasmids and the -184 to +248 reporter gene construct or reporter constructs with the indicated mutations in the identified response elements.

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