Figure 2

DSB formation by plant SPO11 expression in the mei-W68 mus301 double mutant background ( mei-W68¹/ mei-W68¹; mus301^D4/ Df(3L)66C-G28 ). (A) Oocyte nuclei with DSB signals, which were detected by staining γ-H2Av, were scored in stage 2-8 egg chambers. The number of oocyte nuclei with DSB signals was divided by the total number of oocyte nuclei observed. (B) Oocyte nuclei with defective karyosome morphology, which was observed as a non-disc shape, were scored in stage 3-8 egg chambers. The number of oocyte nuclei with defective karyosome morphology was divided by the total number of oocyte nuclei observed. The values of the positive control (bearing the hsp83-mei-W68⁺transgene) for panels A and B were described in the legend to Figure 1C. Transgene (Vector name{gene, insertion #}) [Number of ovaries used for observations]: P{hsp83-AtSPO11-1 cDNA, A5} [7]; P{hsp83-AtSPO11-2 cDNA, M3-1} [3]; P{hsp83-OsSPO11A cDNA, 3M2-3} [3]; P{hsp83-OsSPO11B cDNA, M6-2} [2] and P{hsp83-OsSPO11B cDNA, M7-1} [1]; P{hsp83-OsSPO11A cDNA, 3M2-3} with P{hsp83-OsSPO11B cDNA, F3-2} [2]; P{hsp83-OsSPO11D cDNA, F2-1} [2]; P{hsp83-OsSPO11D^Y213F, M33-F1} [2] and P{hsp83-OsSPO11D^Y213F, F22-F2} [1]. n indicates the number of oocyte nuclei observed. P values from a chi test between the mei-W68 mus301 double mutants without any transgene and those with a plant SPO11 transgene. ***: P < 1E-03; **: 1E-03 <P < 1E-02; *: 1E-02 <P < 5E-02.