Figure 1

Modulation of PR transcriptional activity by SUMO-1 depends on the promoter context. A) schematic of PR-A and PR-B showing the location of hormone dependent phosphorylation sites; the Lys-388 SUMO conjugation site within an IKEE motif; and an acetylation consensus KxKK site (amino acids 638-641). BUS, B-upstream segment; DBD, DNA binding domain; LBD, ligand binding domain. B) HeLa cells were transfected with 2 μg of PRE2-Luc reporter, or C) MMTV-Luc, together with 5-1000 ng of wild-type PR-B or mutant PR-B K388R expression vectors and Renilla-Luc as an internal control. The cells were treated 24 hrs with 10 nM R5020, then harvested and lysed. The extracts were assayed for luciferase activities. Luciferase activity is expressed in relative light units (RLU). Data represent triplicates (± SD). Statistical significance was computed by unpaired student's t test. *p < 0.05.