Figure 3From: Control of progesterone receptor transcriptional synergy by SUMOylation and deSUMOylationThe enhancement of PR transcriptional activity by SENP1 depends on an intact SUMO conjugation site. HeLa cells were transfected with the PRE2-Luc (A and B) or MMTV-Luc (C and D) reporter plasmids in the presence of pSV40-Renilla as internal control along with PR-B (A and C) or PR-B K388R (B and D) expressing vectors, and a Flag-SENP1 expression vector at doses of 20, 50, 100, 200 and 1000 ng of DNA or an empty vector control (-). Cells were treated without (-) or with (+) 10 nM R5020 for 24 hrs before being assayed for luciferase activity as in Figure 1. RLU of wt PR-B in the absence of hormone is set as 1. Statistical significance was computed by unpaired student's t test. *p < 0.05.Back to article page