Figure 7

SRC-1 reverses the inhibitory effect of the HDAC inhibitor TSA on the PR transcriptional activity. A) HeLa cells were transfected with 2 μg of PRE2-luciferase reporters together with 50 ng of a PR-B and Renilla-Luc as an internal control. The cells were treated for 24 hrs with the agonist R5020 (10 nM), without (-) or with increasing amounts of trichostatin A (TSA). B & C) HeLa cells were transfected with 2 μg of PRE2-luciferase reporters together with 50 ng of a PR-B expression vector and Renilla-Luc as an internal control in the absence or the presence (+) of increasing amount of SRC1. The cells were treated for 24 hrs with the agonist R5020 (10 nM), without (-) or with (+) 100 nM (B) or 500 nM (C) of TSA then harvested and lysed. The extracts were assayed for luciferase activities as in Figure 1. (*) Compared with control and (+) compared with TSA treatment.