Figure 10

Inhibition of the MTG16 promoter activity through inhibition of GATA-1 by HERP2. TF-1, HEL, UT-7, or MEG-01 cells were co-transfected with 10 μg MTG16 -668 to -57 bp promoter and the HERP2 plasmid construct. White bars show MTG16 -668 to -57 bp promoter activity without HERP2. Black bars show activity after co-transfection with HERP2, relative to the luciferase activity of the MTG16 -668 to -57 bp promoter in the absence of HERP2 (white bars). All cell lines examined demonstrated repression of GATA-1 driven MTG16 luciferase promoter. Renilla was not affetected by HERP2. The experiments were performed in triplicate. Bars represent the mean and the error bars show SEM.