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Figure 5 | BMC Molecular Biology

Figure 5

From: The leukemia associated nuclear corepressor ETO homologue genes MTG16 and MTGR1 are regulated differently in hematopoietic cells

Figure 5

Functional analysis of putative transcription factor binding sites in the MTG16 promoter. Putative Sp1 (-425), GATA (-301) and MZF-1 (-251) sites in the -668-57 (-668) promoter were individually destroyed by site directed mutagenesis as decribed in Methods. Erythroid HEL/TF-1, erythroid/megakaryocytic UT-7 and megakaryocytic MEG-01cells were transfected and luciferase activity was determined. The pGL3/basic and pGL3/SV40-promoter are used as negative and positive control, respectively. The luciferase activity of mutated MTG16 promoter is normalized against luciferase activity of wildtype MTG16 promoter.

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BMC Molecular Biology

ISSN: 1471-2199

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