Monitoring of reaction dynamics by HPLC. A) The distribution of nucleosides as measured by HPLC depicted as a function of deamination time. The levels of Gs, As and methylated Cs remain constant throughout the reaction. Cs are converted to Us as the deamination progresses. B) Examples of HPLC chromatograms at different deamination times (0 min., 10 min., 15 min. and 30 min.).