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Figure 2 | BMC Molecular Biology

Figure 2

From: Related bifunctional restriction endonuclease-methyltransferase triplets: TspDTI, Tth111II/TthHB27I and TsoI with distinct specificities

Figure 2

SDS/PAGE analysis of the induction pattern of recombinant TspDTI endonuclease. Lane 1, control culture - crude lysate from E. coli expressing the cloned tspDTIRM gene, without induction (OD600 = 0.7); lane 2, control culture after 12 h of cultivation; lane 3, crude lysate from E. coli expressing the cloned gene, before induction (OD600 = 0.7); lane 4, crude lysate from E. coli expressing the cloned gene 3 h after induction; lane 5, crude lysate 6 h after induction; lane 6, crude lysate 12 h after induction; lane 7, purified, homogeneous recombinant TspDTI protein; lane M, protein marker (Thermo Fisher Scientific/Fermentas).

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