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Table 1 Plasmids encoding N- or C-terminal fragments of M.SssI

From: Complementation between inactive fragments of SssI DNA methyltransferase

Plasmid

Res.

N-terminal extension1

C-terminal extension1

pB-Sss[1–189]

Ap

 

EI

pBNH-Sss[1–176]

Ap

MVPGMH6LEC

KLGCFGG

pBNH-Sss[1–222]

Ap

MVPGMH6LEC

ASLAVLADERRFSA

pBNH-Sss[1–239]

Ap

MVPGMH6LEC

SCFGG

pBNH-Sss[1–248]

Ap

MVPGMH6LEC

LAVLADERRFSA

pBNH-Sss[1–257]

Ap

MVPGMH6LEC

EAWLFWRMREDFQPDTD

pBNH-Sss[1–304]

Ap

MVPGMH6LEC

 

pBNH-Sss[1–317]

Ap

MVPGMH6LEC

KLGCFGG

pBNH-Sss[1–318]

Ap

MVPGMH6LEC

KLGCFGG

pBNH-Sss[1–324]

Ap

MVPGMH6LEC

LGCFGG

pBNH-Sss[1–356]

Ap

MVPGMH6LEC

KLGCFGG

pB6ZB-Sss[1–239]

Ap

6-ZFP-B zinc finger domain

SCFGG

pB6ZB-Sss[1–304]

Ap

6-ZFP-B zinc finger domain

 

pOB-Sss[191–386]

Kn

MVP

SH6C

pOB-Sss[231–386]

Kn

MVP

SH6C

pOB-Sss[240–386]

Kn

MV

SH6C

pOB-Sss[241–386]

Kn

M

SH6C

pOB-Sss[242–386]

Kn

MVQ

SH6C

pOB-Sss[246–386]

Kn

MV

SH6C

pOB-Sss[250–386]

Kn

MVH

SH6C

pOB-Sss[254–386]

Kn

MVP

SH6C

pOB-Sss[261–386]

Kn

MV

SH6C

pOB-Sss[279–386]

Kn

M

SH6C

pOB-Sss[304–386]

Kn

M

SH6C

pOB-Sss[316–386]

Kn

MVP

SH6C

pOB-Sss[321–386]

Kn

MVP

SH6C

pSss[191–386]del2

Kn

MVP

SH6C

pSss[241–386]del2

Kn

M

SH6C

pOB-Sss[191–386]SS

Kn

 

SH6C

pOB-Sss[241–386]-6ZA

Kn

M

6-ZFP-A zinc finger domain

pOB-Sss[261–386]-6ZA

Kn

MV

6-ZFP-A zinc finger domain

pBS-CAL75

Ap

  

pBS-Sss6ZA

Ap

 

6-ZFP-A zinc finger domain

  1. Numbers in square brackets specify the M.SssI fragment expressed by the plasmid.
  2. 1 N- and C-terminal extensions were added on purpose (His tag) or are byproducts of the cloning procedure. 2Plasmid lacking the P BAD promoter.