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Table 2 Deoxyoligonucleotides used in this work

From: Complementation between inactive fragments of SssI DNA methyltransferase

Name

Sequence

Properties1

Use

AK183

GAGCTCTTCTTCACAAGAAGA

M.SssI gene sense strand, positions 566-586. Starts with a SacI site

Forward primer for [190-304]

AK184

GCTGCAGTTA AACATAACCTTCTGAATT

M.SssI gene anti-sense strand, positions 912-895, stop codon and PstI site added

Reverse primer for [1-304]

AK185

GCCATG GAATTTAAAAAAACAAAATCA

M.SssI gene sense strand, positions 835-855, NcoI site added

Forward primer for [279-386]

AK186

G CTGCAGTTAGCAGTGATGGTG

M.SssI gene anti-sense strand, stop codon and PstI site added

Reverse primer for His6-Cys-tagged C-terminal fragments

AK188

GCCATG GTTTATGATCCTGAATTTACA

M.SssI gene sense strand, positions 910-930, NcoI site added

Forward primer for [304-386]

AK189

GCCATG GAATTTGTTGAACTACCAAAG

M.SssI gene sense strand, positions 721-741, NcoI site added

Forward primer for [241-386]

AK258

GGCACGCTGACTAAAGGA TTTACCGCACTCGGG

6-ZFP-A gene antisense strand

Mutagenic primer for elimination of a HindIII site

AK259

GCAGCTGTT TCTCCAAAGAAGAAGAGAAAAGT

 

Forward primer for amplification of the 6-ZFP-A gene

AK260

GCTGCAGTTA ACCCAGCTCGCCG

 

Reverse primer for amplification of the 6-ZFP-A gene

  1. Sequences are written in 5’ to 3’ direction. Sequences, which do not correspond to the template DNA, are underlined. 1Nucleotide positions are calculated relative to the first nucleotide of the ATG start codon of the WT M.SssI gene.