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Table 2 Viability of single and double mutant strains in YHR087W and CDC33 under hygromycin 10 μg/mL

From: The Saccharomyces cerevisiae Hot1p regulated gene YHR087W (HGI1) has a role in translation upon high glucose concentration stress

Strain

Viability

BY4741

95,33 ± 8,08

Δyhr087w

73,67 ± 0,58

cdc33 ts

41,71 ± 8,33

Δyhr087w cdc33 ts

27,34 ± 3,45

  1. Dilutions from cultures in exponential phase of growth were plated on YPD or YPD + hygromycin 10 μg/mL plates and the colony forming units appeared 48-72 hours later were counted. Viability was determined as the percentage between the CFU in hygromycin plates and in YPD plates. Application of the t-test to the data obtained for cdc33ts and Δyhr087w cdc33ts mutant strains gave a P value (calculated for one tail using Microsoft Office Excel) of 0.0025.