Figure 1
From: Regulation of GAD65 expression by SMAR1 and p53 upon Streptozotocin treatment
Isolation of DNA fragments bound by SMAR1. A. MEME software based alignment of the SMAR1 binding DNA sequences for the consensus for SMAR1 binding. B. CNBr pull down assay (by consensus and by GAD65 promoter DNA) samples were processed for western blot analysis using SMAR1 specific antibody. PI represents pre-immune and Imm represents the immune complexes. C &D. GST-p53, GST-SMAR1, GST-Rxn5 (DNA binding domain of SMAR1), GST-Rxn6 (Protein binding domain of SMAR1) along with GST and bead only controls was used for in-vitro pull down assays for DNA-protein interactions. The resulting DNA fragments were amplified by PCR using GAD65 (C) and GAD67 (D) specific primers.