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Figure 3 | BMC Molecular Biology

Figure 3

From: Regulation of GAD65 expression by SMAR1 and p53 upon Streptozotocin treatment

Figure 3

SMAR1 upregulates GAD65 expression. A. RT-PCT analysis of GAD65 and p53 upon dose dependent SMAR1 over-expression. Actin was used as loading control. B. Densitometric analysis of the RT-PCR showing ~4 fold increase in p53 and ~7 fold increase in GAD65 mRNA levels upon SMAR1 over-expression. C. SMAR1 was over-expressed in Rin5f cells and samples were processed for western blot analysis 48 hrs post transfection. Figure shows western blot analysis of these samples using GAD65, phospho serine 15 p53 and SMAR1 expression while actin was used as loading control. D. SMAR1 and p53 over expression leads to up-regulation (lanes 2 & 3) while their knock-down abolish the basal expression of GAD65 (lanes 4 & 5). E. DNA binding domain (F5), protein interaction domain (F6) of SMAR1 and full length SMAR1 (FS) transfections led to increased expression of GAD65.

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