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Figure 3 | BMC Molecular Biology

Figure 3

From: A novel 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCR) splice variant with an alternative exon 1 potentially encoding an extended N-terminus

Figure 3

Upregulation of HMGCR transcript variants in HepG2 cells treated with atorvastatin. HepG2 cells were incubated in culture medium with lipoprotein deficient serum (LPDS; non-treated) and 10 μM atorvastatin + LPDS for 24 h (treated). The mRNA levels of (A) HMGCR-1a (B) HMGCR-1b and (C) HMGCR-1c were measured by RT-qPCR using exon1-specific primers and a set of validated reference genes for normalisation. The results are shown as the mean fold-change compared with mRNA levels in non-treated cells. Error bars indicate standard errors (± S.E), n = 5. *Statistically significant differences between non-treated and treated (p < 0.05) were determined using Student’s t-test. †The magnitude of statin-induced upregulation of HMGCR-1b was significantly lower (p < 0.05) than that of HMGCR-1a and HMGCR-1c, respectively, as determined by a paired-sample Student’s t-test.

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BMC Molecular Biology

ISSN: 1471-2199

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