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Figure 3 | BMC Molecular Biology

Figure 3

From: The transcriptional activator ZNF143 is essential for normal development in zebrafish

Figure 3

Rescue of MO phenotypes by co-injection of synthetic zebrafish ZNF143 mRNA. (A) Transcriptional activation of myc-tagged zebrafish ZNF143 deletion proteins in transfected ZF4 cells. Cells were transfected with 200 ng of firefly luciferase reporter plasmid, plus 200 ng of pRL-SV40 renilla luciferase reporter plasmid, plus various ZNF143 effector plasmids as noted in the figure at 5 ng (columns 2, 4, 6) or 25 ng (columns 3, 5, 7). Fold-activation was determined by comparing the firefly/renilla luciferase ratio for each sample to that ratio for the sample shown in column 2. Bar height shows the mean number from different experiments, and error bars report the standard deviation from the mean (when n = 3), or the range (when n = 2). Double asterisks signify a p-value <0.01, and single asterisks signify a p-value <0.05 relative to samples transfected with vector only (lane 2). (B) Relative expression of myc-tagged zebrafish ZNF143 proteins in transfected HEK293 cells. (C) Zebrafish embryos were injected with a combination of "Start" and "5'UTR" MOs along with either no RNA (MOs only), synthetic mRNA encoding wt zebrafish ZNF143 (MOs + FL), or synthetic mRNA encoding zebrafish ZNF143/Δ2-225 (MOs + Δ2-225). Embryos were scored as either dead, wt, or classes 1-6 (see Figure 2 for examples of these classifications) at 48 hpf. Results were averaged from three separate injection experiments for each condition (approximately 100 embryos per injection). The ranges in % wt phenotype for each condition in various experiments were: MOs only: 9-11%; MOs + FL: 69-72%; MOs + Δ2-225: 11-15%.

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