Figure 4

Expression analysis of ZNF143 requirement during embryonic development. In situ hybridizations to detect gata1 (A-D), cmlc2 (E-I), and pax2a (J-N) expression. (A, C, E, J, M) Wild-type. (B, D, F, G, H, I, K, L, N) ZNF143 morphants. (A, B) 16 somite stage, flat mounted embryos, dorsal view, anterior left. Brackets indicate gata1⁺ hematopoietic progenitors. Note reduced expression in the morphant (B). (C, D) 24 hpf, lateral views of tails. Note reduced gata1 expression in the ICM (arrows) of ZNF143 morphants (D) compared to wt (C). (E-G) approximately 27 hpf, dorsal views, anterior up. Note heart laterality in wt (E). (F, G) Two different phenotypes observed in ZNF143 morphants. While they show differences in tube morphogenesis, both lack laterality. (H, I) approximately 37 hpf, dorsal views, anterior up. Two different phenotypes in ZNF143 morphants. (J-L) 16 somite stage, flat mount, dorsal view, anterior left. Compared to the wt (J), ZNF143 morphants (K, L) display reduced pax2a expression in the pronephros (arrowheads), otic vesicles (arrows) and MHB (brackets). Note that expression in these domains is reduced in the morphants even though expression in the optic stalks is similar to wt (asterisks). (M, N) Approximately 27 hpf, dorsal views, anterior left. Expression of pax2a appears largely normal in ZNF143 morphants (N), with the exception of a substantial decrease in expression in hindbrain neurons (arrows). Scale bars: 100 μm in J-L, 200 μm in M, N.