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Table 1 Nomenclature of the S. cerevisiae MultiSite Gateway TM pDEST vectors

From: A MultiSite GatewayTM vector set for the functional analysis of genes in the model Saccharomyces cerevisiae

Name*

Replication

Selectable marker

pMG304

Integrating

TRP1

pMG305

Integrating

LEU2

pMG306

Integrating

URA3

pMG413

CEN

HIS3

pMG414

CEN

TRP1

pMG415

CEN

LEU2

pMG416

CEN

URA3

pMG423

2 μ

HIS3

pMG424

2 μ

TRP1

pMG425

2 μ

LEU2

pMG426

2 μ

URA3

  1. *pMGXYZ where MG indicates the presence of the MultiSite GatewayTM cassette; X stands for the presence (4) or absence (3) of replication determinants on the vector; Y stands for the replication determinant: Integrating (0), CEN (1) or 2 μ (2); Z indicates the auxotrophic selection marker: HIS3 (3), TRP1 (4), LEU2 (5) or URA3 (6).