Figure 1

RecG expression constructs. 1A) RecG expressed as a fusion protein with EGFP. Lettering colors: black: linker amino acids, red: nuclear localization signal, blue: RecG coding sequence, green: EGFP coding sequence. 1B) RecG co-translated with EGFP as two separate polypeptides. Lettering colors: black: linker amino acids, red: nuclear localization signal, blue: RecG coding sequence, brown: strep-tag II, purple: 2a ‘self-cleaving’ peptide, green: EGFP coding sequence. Dotted line represents the division of the precursor polyprotein into two independent polypeptides. The full-length wild-type E. coli RecG polypeptide initiates with the indicated MKGR… peptide sequence and terminates with the indicated …YSNA peptide sequence.