Figure 7
From: Escherichia coli RecG functionally suppresses human Bloom syndrome phenotypes
RecG has no effect on gene cluster instability in cells with normal BLM. Southern blotting detection of the rDNA clusters in BLM+ cells stably expressing higher levels of co-translated RecG/EGFP, “BLM+: RecG 2a”. Cluster lengths resolved up to 1 Mb are shown. Cluster lengths are calibrated by S. cerevisiae chromosome size markers. Open star is the 1 Mb resolution limit of the gel: all clusters larger than 1 Mb co-localize in this region. A clonal cell population is shown in the far left lane of the gel. A collection of subclonal populations each derived from a single cell of this clonal population are shown to the right. Plum circles: relocated major band clusters. Blue braces: former location of major band clusters from the parental clone.