Escherichia coli RecG functionally suppresses human Bloom syndrome phenotypes

BMC Molecular Biology

Table 1 SCE levels and EGFP expression

Cells	SCE/Chr	EGFP	% EGFP+	HPF	Xomes
HeLa	0.09	1.0	0	21	605
HeLa: RecG fuse	0.08	42.6	98	32	835
BLM+	0.10	1.0	0	22	863
BLM+: RecG 2a	0.10	49.3	100	30	999
BLM-: cDNA	0.28	1.0	0	26	1625
BLM-: RecG 2a high	0.33	148	93	33	1861
BLM-: RecG fuse high	0.34	29.8	87	26	1149
BLM-: RecG 2a low	0.68	12.4	92	36	2017
BLM-: EGFP	1.02	27.3	57	48	3013
BLM-: empty vector	1.05	1.0	0	25	1354
BLM-	0.98	1.0	0	16	883
BLM- FC: RecG fuse	0.70	6.4	100	55	1981
BLM- FC	0.91	1.0	0	49	2077
BLM-: RecG fuse med	0.43	30.6	87	29	1925
BLM-: lost RecG fuse	0.86	8.96	1	25	1316

Cells: The stable cell line being investigated.
SCE/Chr: Median number of sister chromatid exchanges observed per chromosome (sister chromatid pair).
EGFP: Relative levels of fluorescence for cells expressing RecG and/or EGFP, relative to cells not expressing EGFP.
% EGFP+: The fraction of cells in a population with above-background green fluorescence due to expression of EGFP.
HPF: The number of high powered microscope fields scored.
Xomes: The total number of sister chromatid pairs scored for exchanges.

ISSN: 1471-2199