Figure 1
From: Transcriptional activation of microRNA-34a by NF-kappa B in human esophageal cancer cells
NF-κB activation correlates with miR-34a expression. (a) EC109 cells were transfected either with empty vector (pcDNA3.1) or p65 expression vector (p65), 48 h after transfection, changes of total p65, nuclear p65 and mature miR-34a levels were individually detected by western blot or qRT-PCR. (b) EC109 cells were transfected either with empty vector (control) or with dominant negative mutant of IκB (DN IκB), nuclear p65 and p50 protein levels and mature miR-34a levels were individually detected by western blot or qRT-PCR.