Figure 2

Overexpression of NF-κB p65 subunit enhances the transcriptional activity of miR-34a. (a) Genomic structure for human miR-34a gene. (b) EC109 cells were transfected with pCDNA3.1 or p65 in combination with the wildtype miR-34a promoter (P1) or promoter with different NF-κB binding site mutated (M1-M3) or promoter with p53 binding site mutated (M53). pRL-TK reporter plasmid was used as transfection control. 48 h later, cells were lysised and luciferase assay were performed as described in Materials and Methods. Error bars represent the standard deviations for three independent experiments. *P < 0.05 * * P < 0.01, t-test versus the P1 reporter vector control.