Figure 1

RNA footprinting of the L30:SECIS complex. A. The structure of the SECIS element from the rat PHGPx mRNA is shown. Boxes and circles indicate nucleotides that are protected from cleavage by RNase T1 and RNase A, respectively. B. The 5’ end-labeled PHGPx SECIS was incubated in the absence or presence of L30 (0.75 or 1.5 μM). The reactions were then partially digested with RNase T1, A, or V1 as indicated. The products were analyzed by denaturing gel electrophoresis. The sequencing (G and C + U) and alkali ladders are shown in the left lanes. The numbers to the left of the gel indicate the positions of G nucleotides using the numbering in (A). The bars on the right indicate the different regions of the SECIS element. The gel is a representative example from 3 independent experiments.