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Figure 2 | BMC Molecular Biology

Figure 2

From: Identification of nucleotides and amino acids that mediate the interaction between ribosomal protein L30 and the SECIS element

Figure 2

The U40C mutation abrogates L30 binding. A. Schematic illustrating the binding sites of SBP2 [14] and L30 (this work) on the SECIS, as determined by RNA footprinting. The position of the U40C point mutation is indicated. B. UV cross-linking experiments were performed using the 32P-labeled wild-type PHGPx SECIS or the U40C mutant RNA, which were incubated with increasing amounts of purified L30 as indicated. After RNase digestion, the products were analyzed by SDS-PAGE and autoradiography.

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