Figure 2From: How to perform RT-qPCR accurately in plant species? A case study on flower colour gene expression in an azalea (Rhododendron simsii hybrids) mapping populationResults of the SPUD assay. Box plot of the Cq values obtained after the analysis of 3 sample types (RNA, cDNA and control) in a SPUD assay. For RNA/cDNA 14 different samples were measured in duplicate, 14 replicates were used for the SPUD control.Back to article page