Name | Sequence | Comments |
---|---|---|
attB-F | GTCATTAATCGCCATTCAGGCTGCGCA | To amplify 400 bp attB site in AseI of pEGFP-N1 |
attB-R | GTCATTAATCTCGGCCTCGACTCTAG | |
attL-F | GGCGAGAAAGGAAGGGAAG | To detect the attL site, 400 bp |
attL-R | ATTAACCCTCACTAAAGGGA | |
attR-F | TCAAAGTAAACGACATGGTG | To detect the attR site,520 bp |
attR-R | CCAACCGCTGTTTGGTCTGC | |
IV-R1 | GCTTATAGATACCGTAGACAT | TAIL-PCR to identify integration site |
IV-R2 | TCCCGTGCTCACCGTGACC | |
IV-R3 | ATCAACTACCGCCACCTCG | |
IV-F2 | CGCCACCTCTGACTTGAGCG | Â |
5113-LF | GTTGGGATTTGGCTCCAGCC | Use with IV-R1 for determination of 5′ end of 5113 pseudo attP site |
5113-RR | TCTTAACACCCCATTGTTCTC | Use with IV-F2 for determination of 3′ end of 5113 pseudo attP site |
5156-LF | GCCTTAATGAGGGCCAGAGC | Use with IV-R1 for determination of 5′ end of 5156 pseudo attP site |
5156-RR | GTTGACCCATTTTCCTGCTCTTCG | Use with IV-F2 for determination of 3′ end of 5156 pseudo attP site |
1015-LF | GTCCTGCCCTGACCCTTTGG | Use with IV-R1 for determination of 5′ end of 1015 pseudo attP site |
1015-RR | GCCAACCTCCAAGTTGTTGG | Use with IV-F2 for determination of 3′ end of 1015 pseudo attP site |
2015-LF | GCAAGGGGTT TTGAAACTGC | Use with IV-R1 for determination of 5′ end of 2015 pseudo attP site |
2015-RR | AAAAGTGCCTCAGAACACCAC | Use with IV-F2 for determination of 3′ end of 2015 pseudo attP site |
5113-pF | TCAAAGTAAACGACATGGTGGAGTGTAGCAGTTCCTAGGC | ABI-REC cloning of 5113 pseudo attP site in pBCPB+ plasmid |
5113-pR | ATTAACCCTCACTAAAGGGATCTTAACACCCCATTGTTCTC | |
5156-pF | TCAAAGTAAACGACATGGTGGACCTCTGCTGTGCCCAGAG | ABI-REC cloning of 5156 pseudo attP site in pBCPB+ plasmid |
5156-pR | ATTAACCCTCACTAAAGGGACCAACCACTGGCTTCCCTCG | |
1015-pF | TCAAAGTAAACGACATGGTGACCCCCACCCTGAGCTACTC | ABI-REC cloning of 1015 pseudo attP site in pBCPB+ plasmid |
1015-pR | ATTAACCCTCACTAAAGGGAACTCCCCAGCAACCAGCTTC | |
2015-pF | TCAAAGTAAACGACATGGTGATGAGACAAGAGGGACCAGG | ABI-REC cloning of 2015 pseudo attP site in pBCPB+ plasmid |
2015-pR | ATTAACCCTCACTAAAGGGAAAAAGTGCCTCAGAACACCAC | |
Pseudo-P1R | CACCATGTCGTTTACTTTGA | ABI-REC cloning of pig pseudo site |
BCPB-S | GCTGCAAGGCGATTAAGTTGGG | Sequencing primer |
EGFP-QF | TGAACCGCATCGAGCTGAAGGG | Real-time PCR primers, 130 bp |
EGFP-QR | ACCTTGATGCCGTTCTTCTGCTTG | |
TFRC-QF | GAGACAGAAACTTTCGAAGC | Real-time PCR primers, 81 bp |
TFRC-QR | GAAGTCTGTGGTATCCAATCC |