From: Reference loci for RT-qPCR analysis of differentiating human embryonic stem cells
Reference | Name | NCBI RefSeq | Function | Amplicon size (bp) | Exon location |
---|---|---|---|---|---|
ACTB* | Beta-Actin | NM_001101 | Cytoskeletal protein | 173 | 7p22 |
B2M§ | Beta-2-microglobulin | NM_004048 | Major histocompatibility complex I component | 86 | 15q21-q22 |
GAPDH* | Glyceraldehyde-3-phosphate dehydrogenase | NM_002046 | Oxidoreductase in glycolysis and gluconeogenesis | 111 | 12p13 |
HMBS* | Hydroxymethyl-bilane synthase | NM_000190 | Porphyrin metabolism and heme synthesis | 64 | 11q23 |
HPRT1* | Hypoxanthine phosphoribosyl-transferase 1 | NM_000194 | Purine synthesis in salvage pathway | 94 | Xq26 |
PPIA* | Peptidylprolyl isomerase A (Cyclophilin A) | NM_021130 | Catalyzation of cis-trans isomerization of proline imidic peptide bonds | 71 | 7p13 |
RPL13A* | Ribosomal protein L13A | NM_012423 | 60S ribosomal subunit structural component | 126 | 19q13 |
SDHA* | Succinate dehydrogenase complex, subunit A | NM_004168 | Electron transporter in citric acid cycle and respiratory chain | 86 | 5p15 |
TBP* | TATA box binding protein | NM_003194 | General transcription factor for RNA polymerase II | 89 | 6q27 |
UBC* | Ubiquitin C | NM_021009 | Involved in protein degradation | 133 | 12q24 |
YWHAZ§ | Tyrosine 3-monooxygenase / tryptophan 5-monooxygenase activation protein, zeta polypeptide | NM_003406 | Signal transduction; binds to phosphorylated serine residues on several signaling molecules | 94 | 2p25 |
AluSq | Alu repeats, subfamily Sq | ||||
POU5F1§ | POU class 5 homeobox 1, Oct4 | NM_002701.4 | Marker for embryonic stem cell pluripotency | 130 | 6p21 |
NANOG* | Nanog homeobox | NM_024865.2 | Marker for embryonic stem cell pluripotency | 109 | 12p13 |