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Figure 3 | BMC Molecular Biology

Figure 3

From: MAR-mediated integration of plasmid vectors for in vivo gene transfer and regulation

Figure 3

Expression of MAR-driven GFP genes after mouse muscle electrotransfer. Wild-type mice muscles were injected with 30 Όg of GFP expression plasmids containing or not a MAR element, or injected with saline for control muscles, as indicated, prior to electrotransfer. Muscles were collected 7 or 56 days after the electrotransfer. Representative transversal muscle sections illustrate the background and GFP fluorescence in green and the extracellular matrix proteins with Wheat Germ Agglutinin separating individual myofibers in red colors (A). The GFP fluorescence of single myofibers was quantified in 4 muscle sections from 2 mice per condition, and they are represented as the average and standard deviation of the GFP fluorescence of individual myofibers, after the subtraction of the background fluorescence values determined from the control muscles (B).

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