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Figure 3 | BMC Molecular Biology

Figure 3

From: An aryl hydrocarbon receptor induces VEGF expression through ATF4 under glucose deprivation in HepG2

Figure 3

AhR regulates activating transcription factor 4 (ATF4) expression in glucose-deprived conditions. A and C: mRNA expression of ATF4. A shows ATF4 expression in glucose-deprived conditions, and C shows ATF4 expression after AhR knockdown. The ATF4 mRNA levels were normalized by the β-actin mRNA level at each point (ATF4/β-actin). The ATF4/β-actin values were calculated relative to the expression level at 0 h (A: the time of medium exchange, C: the time of knockdown), which was set equal to 1. Bars indicate the standard deviation of independent triplicate measurements. * indicates that there is a significant difference (*: P < 0.05, **: P < 0.005). B and D: Western blot analyses using an antibody against ATF4. + and – indicate presence and absence, respectively, of glucose, control RNA, and AhR siRNA. “12 h” and “24 h” in A and B indicate the time following transfer to high or no glucose medium. The RNA and protein samples in C and D were derived from the HepG2 cells at 24 h after the medium exchange.

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