Skip to main content
Figure 3 | BMC Molecular Biology

Figure 3

From: Differential regulation of the rainbow trout (Oncorhynchus mykiss) MT-A gene by nuclear factor interleukin-6 and activator protein-1

Figure 3

Activation of rtMT-A cis-acting elements following exposure to oxidative stress. Luciferase activity in RTH-149 (A) and Hep G2 (B) cells transfected with the indicated rtMT-A enhancer vectors. Cells were exposed to 10 μM PQ (RTH-149) or 100 μM H2O2 (Hep G2). Activities are given as fold induction. All activities were normalized using either the dual luciferase system or β-galactosidase activity. Luciferase activities were analyzed 24 hours post transfection. Results are presented as mean ± SE (n = 4). Statistically significant differences from control levels are indicated by *(p < 0.05); ** (p < 0.01).

Back to article page