Figure 8

Hepatic MT mRNA expression following injection with PQ or PMA. Real-time quantitative PCR was used to determine the relative gene expression levels of the rtMT-A gene. Control fish were injected with 1% DMSO, while experimental fish were injected with 10 μM PQ or PMA and exposed for 24 hours. Results are presented as mean ± SE (n = 5). Expression levels were normalized against EF1α using ∆∆CT method. Statistically significant differences from control levels are indicated by *(p < 0.05); ** (p < 0.01).