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Table 1 Oligonucleotides used for EMSA competition binding assays

From: Differential regulation of the rainbow trout (Oncorhynchus mykiss) MT-A gene by nuclear factor interleukin-6 and activator protein-1

Primer

Sequence

AP1-1,2

TGG TAT GAC ACA GCT C AA TTA CTC AAG CAG

AP1-1*,2

TGG TAT GAC ACA GCT C AA TTA ATT AAG CAG

AP1-1,2*

TGG TAT AAT ACA GCT CAA TTA CTC AAG CAG

AP1-1*,2*

TGG TAT AAT ACA GCT CAA TTA ATT AAG CAG

AP1-3,4

CTG GTA CTG TCA GTG ACT ATT T

  1. The different AP11,2 and AP13,4 oligonucleotides corresponds to position (-814 to -785) and (-867 to -836) of the rtMT-A promoter respectively. The asterisk (*) indicates a mutated cis-element.
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BMC Molecular Biology

ISSN: 1471-2199

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