Identification of a common reference gene pair for qPCR in human mesenchymal stromal cells from different tissue sources treated with VEGF

Table 2 Step two of elimination, showing ASC and BMSC reference gene intergroup variations

Difference in mean expression of each gene between treated and untreated groups is shown as standard deviations (SD) in unit cycles. Genes were excluded at a cutoff value above 0.2. Those with a SD below the threshold value (*) were included considered for further processing. 18S rRNA: 18S ribosomal RNA, ACTB: beta-actin, EF1-a: Elongation factor 1-alpha, GAPDH: gluco phosphate dehydrogenase, GUSB: Beta-glucuronidase, PPIA: Peptidylprolyl isomerase A, RPL13a: Ribosomal protein L13-alpha, TBP: TATA box binding protein, YWHAZ: Tyrosine 3/tryptophan 5-monooxygenase activation protein. Genes not indicated with a star (*) were deactivated in GenEx.

ISSN: 1471-2199