Figure 3
Identification of DZIP1-associated mRNAs in HeLa cells. (A) Western blot of HeLa cell proteins immunoprecipitated with a specific anti-DZIP1 antibody and a control IgG, probed with a specific anti-DZIP1 antibody; (B) mRNAs associated with DZIP1. Microarray analysis of the immunoprecipitated RNA fractions. Rows correspond to individual transcripts and the color code indicates the degree of enrichment. Three independent assays, with anti-DZIP1 antibody and negative controls, are shown. (C) Quantitative RT-PCR analysis of transcript levels in DZIP1-IPs (DZIP1 immunoprecipitate) versus negative IPs (IP-Control), normalized to concentration. Enrichment of the following mRNAs was analyzed: IFT80, PUM1, SNX2, BRD8, PTCH, DISP1, NPC1, CSNK1E, MYO5B, CEP164, STK36 and ASH1L. The mean of technical triplicates is shown. (D) Quantitative RT-PCR of transcript levels in DZIP1-GFP IPs versus negative IPs (IP-GFP), normalized to concentration. Enrichment of the following mRNAs was analyzed: IFT80, PUM1, SNX2, BRD8, PTCH, DISP1, NPC1, CSNK1E, MYO5B, CEP164, STK36 and ASH1L. The mean of technical triplicates is shown.