Exploring the transcription activator-like effectors scaffold versatility to expand the toolbox of designer nucleases

Table 1 Activities of the TtH and HtH nuclease architectures at their endogenous cognate targets

Architectures and endogenous loci			Total events [%]	Total events [nb]	Insertion [nb]	Deletion [nb]	Wt [nb]	Reads [nb]
TtH	DMD	NC_000023.10: 32,364,567-32,364,620	1.8	193	58	141	10538	10731
TtH	DMD	NC_000023.10: 32,364,567-32,364,620	1.8	193	(16)	(48)	10538	10731
ctrl	DMD	NC_000023.10: 32,364,567-32,364,620	0	0	0	0	7837	7837
TtH	RAG1	NC_000011.9: 36,594,622-36,594,675	2.6	59	18	41	2221	2280
TtH	RAG1	NC_000011.9: 36,594,622-36,594,675	2.6	59	(7)	(24)	2221	2280
ctrl	RAG1	NC_000011.9: 36,594,622-36,594,675	0	0	0	0	5494	5494
TtH	FUT8	NW_003613860.1 673,480-673,533	8.7	459	134	330	4817	5276
TtH	FUT8	NW_003613860.1 673,480-673,533	8.7	459	(60)	(80)	4817	5276
ctrl	FUT8	NW_003613860.1 673,480-673,533	0.033	2	1	1	6129	6131
HtH	DMD	NC_000023.10: 32,364,534-32,364,592	0.01	1	1	0	9147	9148
ctrl	DMD	NC_000023.10: 32,364,534-32,364,592	0.01	1	1	0	9540	9541
HtH	RAG1	NC_000011.9: 36,594,571-36,594,629	1.2	10	5	6	861	871
HtH	RAG1	NC_000011.9: 36,594,571-36,594,629	1.2	10	(3)	(6)	861	871
ctrl	RAG1	NC_000011.9: 36,594,571-36,594,629	0.051	2	1	1	3913	3915
HtH	FUT8	NW_003613860.1: 673,442-673,500	30.5	1791	1191	613	4088	5879
HtH	FUT8	NW_003613860.1: 673,442-673,500	30.5	1791	(64)	(159)	4088	5879
ctrl	FUT8	NW_003613860.1: 673,442-673,500	0	0	0	0	7223	7223

The number of unique events (considering the size and position) is indicated in brackets. Control (ctrl) indicates a tranfection with an empty vector plasmid.

ISSN: 1471-2199