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Figure 5 | BMC Molecular Biology

Figure 5

From: Egr-1 regulates the transcription of NGX6 gene through a Sp1/Egr-1 overlapping site in the promoter

Figure 5

Transcriptional factor Egr-1 increases NGX6 promoter activity in SW620 cells. 0.5 μg pGL3-86/+12 vector was cotransfected with 0.5 μg SV40 β-galactosidase vector and various amount pCMV-HA/Egr-1 into SW620 cells by Lipofectamine 2000 Reagent. Luciferase activity was measured in cell extracts after transfection for 48 h. Then β-galactosidase was used for for normalizing transfection efficiency. Data are expressed as mean ± SE of three independent experiments.

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