Figure 2

Primer sequences for amplifying the different USER-Bricks, specifying template, product size and compatible USER-Brick fragments. PCR templates: A = pAg1-H3, pRF-HU2 [15], B = pPK2 or pPZP-201BK [16], C = pANT-hyg(R) [17] or pCSN43 (Fungal Genetics Stock Center), D = pAN7-1 [18], E = pSM334 [19], F = pBARKS1 [20], G = A. nidulans genomic DNA or pRF-HUE, pRF-HU2E [15], H = pWJ1350 [21] or plasmids derived from the original Discosoma sp. study [22]. In primer sequences: U = 2-deoxyuridine.