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Figure 3 | BMC Molecular Biology

Figure 3

From: Genetic transformation of Fusarium avenaceum by Agrobacterium tumefaciens mediated transformation and the development of a USER-Brick vector construction system

Figure 3

Primer overhangs that should be added to the 5’ end of the gene specific primers to allow for construction of the specified types of vector constructs. A = natural promoter regions of the target gene; B = the terminator region of the gene and C: for in locus overexpression experiments the first 1500 bp of the gene so that the AU in the forward primer (HRS3, CDS-F and HRS3f) is part of the start codon, and for ectopic expression experiments so that the entire coding sequence and terminator are amplified.

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