Skip to main content

Erratum to: Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermussp. enzyme family

The Original Article was published on 29 May 2009

Correction

The TspGWI restriction endonuclease, which originates from thermophilic Thermus sp. GW, was cloned previously [1] in Escherichia coli (E. coli) using a method employing aa sequencing of proteolytic fragments N-termini, followed by a combination of sequencing of degenerated, inverse and standard PCR products and clones containing the tspGWIRM gene. A combination of these methods yielded a sequence of 3688 bp, comprising an entire TspGWI Open Reading Frame (ORF: 3291 bp) and flanking sequences [GenBank: EF095488, ABO26710]. However, more recent resequencing of the tspGWIRM gene with the use of genomic Thermus sp. GW DNA as a template in PCR, obtained with primers external to ORF, has revealed a sequencing error. This resulted in a frameshift of 233 aa, starting at aa 740 and returning to the original ORF at aa 973. The frameshift was located within the 3′-terminal portion of the gene, coding for a Target Recognition Domain (TRD). The nt sequence of the tspGWIRM gene was corrected at 2217 bp (C insertion) and further downstream at 2915 nt (C deletion), restoring the ORF (Additional file one (Additional file 1 here) and Figure five (Figure 1 here) corrected).

Figure 1
figure 1

Sequence alignment between TspGWI and its close homologues in REBASE.

New figures have been prepared using the corrected DNA and aa sequences (Figure five (Figure 1 here) and Additional file one (Additional file 1 here)). Data concerning corrected nt and aa TspGWI sequence have been deposited in GenBank [GenBank: KJ730526].

The correction was located within a variable TRD segment, thus the conclusions of the bioinformatics sequence analysis must be slightly modified compared to our originally published results [1]. The corrected sequence of TspGWI shows similarity to the TaqII sequence and residues 660–960 in TspGWI that were originally (in the previous version) predicted to be intrinsically disordered, were predicted to be precisely ordered in the corrected version of the sequence (data not shown).

The DNA sequence and the predicted amino acid sequence of the 120.2 kDa TspGWI protein is indicated in capital letters. DNA sequences of flanking regions are indicated in italics. The ATG start codon and TGA stop codon are emboldened and underlined. Potential TspGWI Ribosome Binding Sites (RBS) are emboldened, underlined italics.

References

  1. Żylicz-Stachula A, Bujnicki JM, Skowron PM: Cloning and analysis of bifunctional DNA methyltransferase/nuclease TspGWI, the prototype of a Thermus sp. family. BMC Mol Biol. 2009, 10: 52-10.1186/1471-2199-10-52.

    Article  PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

This work was supported by DS/530-8640-D509-14 (PMS, AZS), Gdansk University, Chemistry Department DS fund. JMB was supported by the statutory funds of the IIMCB and the UAM.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Piotr M Skowron.

Additional information

The online version of the original article can be found at 10.1186/1471-2199-10-52

Electronic supplementary material

Authors’ original submitted files for images

Below are the links to the authors’ original submitted files for images.

Authors’ original file for figure 1

Rights and permissions

Open Access  This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.

The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.

The Creative Commons Public Domain Dedication waiver (https://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zylicz-Stachula, A., Bujnicki, J.M. & Skowron, P.M. Erratum to: Cloning and analysis of a bifunctional methyltransferase/restriction endonuclease TspGWI, the prototype of a Thermussp. enzyme family. BMC Molecular Biol 15, 16 (2014). https://0-doi-org.brum.beds.ac.uk/10.1186/1471-2199-15-16

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://0-doi-org.brum.beds.ac.uk/10.1186/1471-2199-15-16