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Figure 5 | BMC Molecular Biology

Figure 5

From: Establishment of a quantitative PCR system for discriminating chitinase-like proteins: catalytically inactive breast regression protein-39 and Ym1 are constitutive genes in mouse lung

Figure 5

Validation of a qPCR system for the analysis of mouse tissues. The analyzed cDNAs were the following: (A), BRP-39; (B), Ym1; (C), Ym2; (D), Chit1; (E), AMCase; (F), GAPDH; (G), β-actin; and (H), pepsinogen C. Standard curves were obtained using the mouse Refs/CLPs standard DNA with pGEM-T Easy containing the eight mouse cDNA fragments (red closed circles). In addition, the quantification of the mouse entire coding cDNA was performed using the primer pairs for each gene. The target cDNA was amplified from a dilution of the entire coding cDNA with a known concentration and subsequently analyzed as an unknown sample (blue closed rhombuses). Equal quantities were obtained for each tested dilution of the standard curve and entire coding cDNA. Data are expressed as mean ± standard deviation (SD) of three measurements.

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