Inverted terminal repeats of adeno-associated virus decrease random integration of a gene targeting fragment in Saccharomyces cerevisiae

Table 1 Effect of the AAV ITR sequence on gene targeting and random integration in yeast

Plasmid	Gene targeting	Random integration	GT/RI
ITR-lys2URA3lys2-ITR	31.0 ± 11.3 (7025)	0.62 ± 0.37 (487)	50.2
lys2URA3lys2	35.4 ± 10.4 (6960)	1.82 ± 0.41 (3420)*	19.4

Yeast was transformed with Pvu II- or Xba I-restricted pAAVLUL as reported in Materials and Methods. Gene targeting and random integration events are reported as number of URA3⁺lys2^-colonies /10³ transformants per μg of plasmid DNA and URA3⁺LYS2⁺colonies /10³ transformants per μg of plasmid DNA, respectively. The number of transformants per μg of DNA was determined by transformation with 1 μg of episomal plasmid DNA. Results are the mean of four independent experiments ± standard deviation. The number of total colonies is indicated in parentheses. Statistical analysis was performed using Student’s t- test. *p = 0.0048 vs ITR-lys2URA3lys2-ITR.

ISSN: 1471-2199