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Table 3 Effect of TATA-binding protein on the transcription efficiency

From: A novel, non-radioactive eukaryotic in vitro transcription assay for sensitive quantification of RNA polymerase II activity

Template Reaction Signal (RLU average) % RNA synthesis* % RNA synthesis
TATA mutant vs. standard**
HS-DNA_std Positive 1306740 100% ± 3% 100% ± 3%
Negative 9768   
α-amanitin 200 nM 161585 12% ± 1%  
HS-DNA_mut Positive 985650 100% ± 10% 75% ± 7%
Negative 11795   
α-amanitin 200 nM 130255 12% ± 1%  
  1. Transcription reactions were performed using the standard DNA template (HS-DNA_std) or a TATA-box mutated template (HS-DNA_mut). Transcription efficacy was determined by QuantiGene quantification.
  2. *Compared to respective average positive control, less the respective negative controls.
  3. **Compared to average positive control on standard template, less the respective negative controls.