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Table 3 Evaluation by conventional statistical analysis of HKG levels and stability

From: Validation of housekeeping gene and impact on normalized gene expression in clear cell Renal Cell Carcinoma: critical reassessment of YBX3/ZONAB/CSDA expression

 

Wilcoxon p value

CtCV intraN (%)

CtCV intraT (%)

CtCV Tot (%)

18S

0.042*

15.94

29.15

24.52

β -Act

0.391

3.34

9.11

6.85

GAPDH

0.017*

7.71

13.13

12.72

B2M

0.042*

6.82

12.54

10.96

RP2

0.194

3.59

6.59

5.45

PPIA

0.626

3.58

6.66

5.36

RPL27

0.035*

3.97

7.06

6.26

RPS13

0.715

3.55

7.56

5.90

  1. Statistical differences between Ct for tumoral versus normal tissue were assessed by non-parametric paired Wilcoxon test. Highest p values indicate best candidates; *(p < 0.05) indicates unsuitable HKG. Coefficients of variation (CtCV) are given for normal (intraN), tumoral (intraT) and all confounded (Tot) samples. Lower values reflect lesser dispersion and better stability.