Table 1 Structure and sequences of c-myc antisense and control oligonucleotides
From: Expression of c-myc is not critical for cell proliferation in established human leukemia lines
Methylphosphonodiester / phosphodiester chimeric oligodeoxynucleotides | |
|---|---|
5'F-MD685AS | 5'–Fluos-C/T/G/C/T/G/T-C-G-T-T-G-A-G-A/G/G/G/T/A–3' |
AS targeting 1147–1166 | |
5'F-MD586iAS | 5'–Fluos-A/T/G/G/G/A-G-A-G-T-T-G-C-T/G/T/C/G/T/C–3' |
Inverted AS control | |
5'F-MD586S | 5'–Fluos-T/A/C/C/C/T-C-T-C-A-A-C-G-A/C/A/G/C/A/G–3' |
Sense 1147–1166 control | |
5'F-MD757AS | 5'–Fluos-C/T/G/C/T/G/T/C-G-T-T-G-A/G/A/G/G/G/T/A–3' |
AS targeting 1147–1166 | |
CpG oligodeoxynucleotide 5-mer | |
CGTTG | 5 '–Fluos-C-G-T-T-G-ProOH–3' |
CpG motif present in 1147–1166 AS and 559–573 AS | |
Morpholino oligonucleotide analogues | |
M28AS | 5'–Fluos-GGC AUC GUC GCG GGA GGC UGC UGG AGC G–3' |
AS targeting from within Intron 1 to 563 | |
M28NS | 5'–Fluos-CAG GCC UUG UGG ACG GCG GUU CGC GAG G–3' |
Nonsense control | |
M28S | 5'–Fluos-CGC UCC AGC AGC CUC CCG CGA CGA UGC C–3' |
Sense from within Intron 1 to 563 control | |