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Table 1 Effects of substitutions of Val29 on translational repression. The numbers shown are the relative β-galactosidase activities produced by bacteria containing the indicated plasmid constructs.

From: Asymmetric interactions in the adenosine-binding pockets of the MS2 coat protein dimer

pCT119

2

p2CTdl-13

1

pUCter3

100

2V29A

97

2V29F

6

2V29I

1

2V29L

2

2V29W

82

2V29Y

7

V29A-WT

7

V29F-WT

2

V29I-WT

1

V29L-WT

2

V29W-WT

3

V29Y-WT

2

V29A-Y85H

7

V29F-Y85H

3

V29I-Y85H

1

V29L-Y85H

2

V29W-Y85H

15

V29Y-Y85H

2

V29A-T91I

9

V29F-T91I

3

V29I-T91I

2

V29L-T91I

4

V29W-T91I

4

V29Y-T91I

3