Figure 1From: Loss of cellular adhesion to matrix induces p53-independent expression of PTEN tumor suppressorPTEN protein levels in suspension culture cells MDAH or 10T1/2 cells were transferred to suspension culture conditions for the times indicated. Total cellular lysate was prepared and analyzed by Western blot with PTEN specific antibodies. In addition, different cell cycle-regulatory proteins were analyzed in parallel as indicated. The top panel shows lysates from 10T1/2 cells, whereas MDAH cells are represented in the bottom panels.Back to article page