Figure 2

Blood concentrations achieved by muscle electroporation. Endostatin and mhATF-BPTI plasma levels (red lines) and muscle luciferase activity (black lines) in C57BL/6 mice. The animals were sacrificed 4, 7, and 14 days after treatment to determine the luciferase activity in the injected muscle and plasma levels of endostatin or ATF-BPTI. Five animals per group and data are expressed as means ± SD. A: the mice received 50 μg pAdapt hEndo +/- 50 μg pAdapt Luc intramuscularly followed by no electroporation (circles) or by a 200 V/cm pulse delivery (squares). B: the mice received pAdapt mhATF-BPTI +/- 50 μg pAdapt Luc intramuscularly followed by no electroporation (circles) or by a 200 V/cm pulse delivery (squares). The plasma levels of mhATF-BPTI injected without electroporation were below the detection limit. The injection of two plasmids together did not influence the level of expression of either the luciferase plasmid or that of the plasmid encoding mhATF-BPTI or endostatin.